A method for analysis of cell to cell transmission of Theileria sergenti piroplasms.
نویسندگان
چکیده
We tried to develop an experimental system for quantifying piroplasm transmission in Theileria sergenti infection using a SCID mouse model, of which red blood cells (RBC) had been substituted with bovine (Bo). A mouse hybridoma cell producing antibody against Holstein RBC was established for distinguishing Holstein RBC from Japanese Black RBC by a immuno-staining method. T. sergenti piroplasms in RBC were stained by 4',6-diamidino-2-phenylindole. With the aid of these techniques the piroplasm transmission from one to another race of Bo-RBCs could successfully be quantified in SCID mice.
منابع مشابه
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ورودعنوان ژورنال:
- The Journal of veterinary medical science
دوره 60 1 شماره
صفحات -
تاریخ انتشار 1998